Mannosyl, glucosyl or galactosyl liposomes to improve resveratrol efficacy against Methicillin Resistant Staphylococcus aureus biofilm

Novel cationic glycoliposomes, composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), cholesterol (Chol) and glycoamphiphiles featuring a galactosyl, mannosyl or glucosyl moiety have been investigated for the targeted delivery trans-resveratrol (RSV), Quorum Sensing Inhibitor (QSI), to Methicillin Resistant Staphylococcus Aureus (MRSA) biofilms. All glycosylated formulations show 10–20 % reduction their hydrodynamic size high positive increase in ?-potential (20÷27mV), with respect almost neutral DOPC/Chol liposomes (-3.7 mV). RSV is entrapped Entrapment Efficiency (EE%), containing amphiphiles showing higher values EE% (79–90 %) than those DOPC/CHOL (65 %). In all liposomal formulations, inclusion causes decrease ?-potential, which particularly evident negative value (-14.8 This probably due ionization small percentage molecules that point towards lipid/water interface, as reported literature. Greater antioxidant activity found when embedded liposomes, rather liposomes. finding suggests different distribution lipid membrane enclosing amphiphiles, favor an external exposure RSV. Biological assays carried out monitor demolition effect RSV-loaded on mature biofilm MRSA presence essential take place matrix. particular, RSV-galactosylated are most effective destroying even at concentration (0.019 mM) sixty times lower MIC (1.2 mM). work demonstrates, first time, how functionalization glycosydic residues can enhance liposome performances QSI nanocarriers treatment associated infections.